Students should be able to use techniques of cellular and molecular biology in an appropriate way and to propose some strategies when facing a biological question in research.
The main objective is to confront students to many modern experimental techniques and approaches currently used in Cell and Molecular Biology laboratories.
Transgenic and KO animal models are also presented.
The goal is to stimulate students to adopt a critical mind when analyzing the advantages and limitations of these various techniques.
See table of content
Prof. Thierry Arnould
1) Cytometry and FACS analysis
2) Cell transfection methods in eukaryotic cells
3) Gene silencing : siRNA, miRNA.
Prof. Henri-François Renard
Prof. Olivier De Backer
1) Introduction : les organismes modifiés génétiquement ; la souris comme organisme modèle
2) Transgenèse d’addition vs ciblée
3) Méthodes de transgenèse ciblée chez la souris : manipulation de cellules ES et du zygote. Edition du génome par le système CRISPR/Cas9.
4) Usages des recombinases site-spécifiques (Cre, Flp) en transgenèse
5) Traçage de lignées cellulaires
6) Systèmes d'expression inductible
The students have to take and pass an oral exam with every single Professor. Students have time (± 60 min) to prepare a question/professor in relation with their respective topics. 4 to 5 students start at the beginning of the day and then enter every 30 min. Professor will particularly put the accent on important concepts and will specially pay attention to the way of thinking, comprehension and reflection more than memory of details.
In the case of strong failure (note of 7 or lower) in one or several parts of this course, a note of 6/20 will appear on the deliberation sheet. Therefore the student will need to present in the second session the part(s) in which a failure was reported, keep the notes for the other parts of the courses acquired at the first session.
Slides and research articles derived from the literature