Learning outcomes

The student will acquire a good knowledge of the principal techniques of bases of biochemistry but also of cellular and molecular biology. 
The student will have to be able to choose an experimental approach and to justify it according to the advantages and limitations presented by the various analyzed techniques. 
The student will be able to interpret graphic results of experiments using the techniques seen during the different lectures and to understand experimental protocols.

 

Goals

To explain the principles of many techniques by showing the advantages and the limitations of these techniques. 
To familiarize the student with the techniques regularly used in the scientific literature of the life sciences.

Content

The biochemical course of techniques examinesthe methods of measurement and analysis used inthe laboratories which call on biochemistry like the university research, medical and pharmaceutical studies.

One exposes to it the theoretical aspect of these techniques.

The student learns the basic techniques: chromatographiescentrifugations and cell fractionation, purification of enzymes and DNA, gene expression analysis and molecular interactions by immunoprécipitations (Co-IP, Chip, Tap-Tag,Sweater-down assay), FRET, of molecular mobility (FRAP), measurement of the activity transcription factors (EMSA, reporter gene) work with the antibodiesspectrophotometric and radioactive assays.

 

Table of contents

Biochemical Assays, 

Preparations of samples and recalls 
Techniques of centrifugation 
Affinity chromatrography
Interactions protein-protein 
Techniques related to the analysis of the genic expression 
Nucleic acid-protein interactions

Cell transfection

Exercices

Practical courses : 25 h

The participation to all practical courses is mandatory to register and pass the exam on the theory.

 

 

Assessment method

Oral exam :

Questions: the student draws a card of questions related to the matter of the course (usually 2 major questions). 

Preparation: the student prepares a framework of answers.

He has between 60 and 90 minutes for preparation.

Four to five students enter the room of examination at each beginning of half-day of interrogation.

Seven to eight students can be simultaneously preparing their examination in the same room.

Oral interrogation: the student exposes his responses to the question while resting about the groundwork which it prepared.

Derived questions intervene under examination/discussion. The duration of  examination generally lies between

15 and 30 minutes in front of the professor.

The final note is modulated by the note btained by the student for the pratical courses (4 points out of 20 = 20 % of the final evaluation).

Within the same academic year, this note for pratical courses is taken into account at each evalaution period.

 

Sources, references and any support material

Slides, books and papers clearly suggested at the beginning of the course.

Several research articles developping the different techniques will also be loaded on Webcampus.

Language of instruction

Français
Training Study programme Block Credits Mandatory
Bachelier en sciences biologiques Standard 0 2
Bachelier en sciences biologiques Standard 3 2