Students should be able to use techniques of cellular and molecular biology in an appropriate way and to propose some strategies when facing a biological question in research.
The main objective is to confront students to many modern experimental techniques and approaches currently used in Cell and Molecular Biology laboratories.
Transgenic and KO animal models are also presented.
The goal is to stimulate students to adopt a critical mind when analyzing the advantages and limitations of these various techniques.
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Prof. Thierry Arnould
1) Cytometry and FACS analysis
2) Techniques tof study the Extracellular Vesicles or other technologies that are recent and at the edge in the cellular and molecular biology field.
3) Gene silencing siRNA, miRNA.
Prof. Henri-François Renard
Prof. Olivier De Backer
1) Introduction : les organismes modifiés génétiquement ; la souris comme organisme modèle
2) Transgenèse d’addition vs ciblée
3) Méthodes de transgenèse ciblée chez la souris : manipulation de cellules ES et du zygote. Edition du génome par le système CRISPR/Cas9.
4) Usages des recombinases site-spécifiques (Cre, Flp) en transgenèse
5) Traçage de lignées cellulaires
6) Systèmes d'expression inductible
The programme has been established in a consensual way between the 3 Professors in order to cover major and many experimental techniques, equipments and strategies used in modern biology. The course has been planned to give tools and keys to students in order to allow them to read and to understand scientific papers as well as to give them basic knowledge to plan a research project. Lectures supported by various pedagogical tools are accompanied by extra readings of scientific papers.
These papers are analyzed in a critical manner by the teachers.
The students have to take and pass an oral exam with every single Professor. Students have time (± 60 min) to prepare a question/professor in relation with their respective topics. 4 to 5 students start at the beginning of the day and then enter every 30 min. Professor will particularly put the accent on important concepts and will specially pay attention to the way of thinking, comprehension and reflection more than memory of details.
In the case of strong failure (note of 7 or lower) in one or several parts of this course, a note of 6/20 will appear on the deliberation sheet. Therefore the student will need to present in the second session the part(s) in which a failure was reported, keep the notes for the other parts of the courses acquired at the first session.
Slides and research articles derived from the literature
| Training | Study programme | Block | Credits | Mandatory |
|---|---|---|---|---|
| Master in Biochemistry and Molecular and Cell Biology, Research focus | Standard | 0 | 3 | |
| Master in Biology | Standard | 0 | 3 | |
| Master in Biochemistry and Molecular and Cell Biology, Teaching focus | Standard | 0 | 3 | |
| Master in Biochemistry and Molecular and Cell Biology, Professional focus | Standard | 0 | 3 | |
| Master in Biochemistry and Molecular and Cell Biology, Research focus | Standard | 1 | 3 | |
| Master in Biology | Standard | 1 | 3 | |
| Master in Biochemistry and Molecular and Cell Biology, Teaching focus | Standard | 1 | 3 | |
| Master in Biochemistry and Molecular and Cell Biology, Professional focus | Standard | 1 | 3 |