1. The student will have acquired the basic principles of protein biochemistry, i.e. how the physico-chemical properties of amino acids and peptide chains condition the structure and therefore the function of proteins.
2. The student will have acquired a global vision of the different means of regulation of the enzymatic activity, and of the methods allowing to measure this activity.
3. the student will be able to propose methods for the purification and analysis of a mixture of proteins
4. The student will have acquired basic knowledge of simple sugars and their derivatives, the main polysaccharides, and glycoconjugates
5. The student will have a global vision of the principles governing cellular metabolism and the main regulations. These principles are highlighted through carbohydrate metabolism: glycolysis, glycogen metabolism, Krebs cycle and phosphorylating oxidations
1. To acquire the basic principles of protein biochemistry: how the physico-chemical properties of amino acids and peptide chains condition the structure and therefore the function of proteins.
2. Understand the principles governing enzyme activity and kinetics for simple reactions, and the modes of regulation
3. Acquire the principles needed to purify and analyse a mixture of proteins
4. To acquire the basic principles of sugar biochemistry.
5. Knowledge of glycolysis, glycogen metabolism, the Krebs cycle and phosphorylating oxidations
The biochemistry course introduces the basic concepts of biochemistry at the level of the structure of proteins in relation to their function, highlighting the mechanisms used to explain the exceptional properties of enzymes, namely an extremely high catalytic power and a very high specificity. These properties give living organisms their remarkable control over matter and energy. The basics of enzymatic activity and enzymatic kinetics of simple reactions (with one substrate) are seen, as well as various modes of regulation of enzymatic activity. A part of the course is devoted to the biochemistry of mono- and polysaccharides and their conjugation to proteins. The metabolic pathways related to glucose catabolism are studied: glycolysis, glycogenesis and glycogenolysis, Krebs cycle and phosphorylating oxidations. The emphasis is on enzymatic regulation in a metabolic context.
The biochemistry course builds progressively on the foundations of chemistry and introduces the basic concepts of biochemistry at the level of the structure of proteins in relation to their function. An essential part of the course is to understand the forces that determine the folding of peptide chains and the mechanisms involved in explaining the catalytic mechanisms of enzymes. Several protein purification and analysis methods are available. A minimum of knowledge is therefore required, but the student will be assessed mainly on the level of basic concepts and explanatory reasoning. The basic principles of biochemistry are therefore a condition for success. The student must also be able to justify his or her answers during the oral examination where the question of why will often come up. The overall score consists of 16 points for the theory assessment and 4 points for the practical assessment. The assessment of the practical work includes : - Entry tests carried out before each session (except for the introductory session) to ensure that the student has properly prepared for the practical session. - writing a laboratory report - an oral examination, held on the same day as the theoretical examination, to assess the student's mastery of : • The different stages of the techniques used • the significance of the results obtained (raw and after calculations) • calculations made on the basis of raw data • extensive interpretation of the data obtained. Any fraud, even in connection with the practical work, will be sanctioned by a mark of 0/20.
Lehninger - Principles of Biochemistry (8th edition) : M. Cox and D.L. Nelson.
| Training | Study programme | Block | Credits | Mandatory |
|---|---|---|---|---|
| Bachelier en sciences chimiques | Standard | 0 | 4 | |
| Bachelier en sciences chimiques | Standard | 2 | 4 |