Learning outcomes

After completing this course, students will master several techniques used in routine in a research laboratory. These techniques include: cloning, bacteria transformation, mini-preparation of plasmids, PCR, RT-qPCR, western blot, immunohistochemistry, and cell fractionation.

 

Students will learn new techniques including next-generation sequencing, taqman protein assay and digital PCR.

Goals

The major aim of this course is to train students in basic laboratory techniques and to prepare them for the laboratory internship.

Content

  1. RT-qPCR: methods et applications
  2. Digital PCR
  3. PCR Protein Assay
  4. Next-Generation Sequencing

Exercices

Practicals spanning techniques of molecular and cellular biology

Assessment method

Oral exam: /10

Practicals: a final report is graded on 10

The exact modalities of the evaluation are likely to be modified during the preparation of the examination schedules, depending on the practical constraints with which the faculty administration may be confronted, or in the event of illness / force majeure / encroachment with an internship, or because of the health situation related to the coronavirus.

Sources, references and any support material

PowerPoint presentations, textbook of practicals, research and review articles.

Language of instruction

Français