Learning outcomes

Theoretical and practical approach of standard methods in cell and tissue biology in order to identify their field of application, usefulness and limitations.

Goals

To understand, perform and describe basic experiments in preparation for the master thesis. Therefore, access is restricted to students in their final bachelor year.

Content

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Table of contents

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Exercices

Students achieve a practical approach of several basic methods and learn how to write a lab report.

Assessment method

Oral or written exam

Lab reports are taken into account for attribution of grade

Sources, references and any support material

Molecular cloning : A laboratory manual. Green and sambrook, 4th edition, Cold Spring Harbor Laboratory Press 2012.

Culture of animal cells : A manual of basic techniques and specialized applications. R.Ian Freshney, 7th edition, Wiley and sons, Hoboken New Jersey 2016

Language of instruction

Français
Training Study programme Block Credits Mandatory
Bachelier en sciences biomédicales Standard 0 4
Bachelier en sciences biomédicales Standard 3 4