In many molecular mechanisms, the three-dimensional structure of proteins allows to explain fundamental processes such as the binding of partners, catalysis or allostery. At the end of the course, the student should be able to analyse a three-dimensional (3D) structure of proteins, to have a critical point of view about the validity of the proposed structure, and to familiarized with the techniques of 3D structure determination and prediction.
Familiarize the student with tridimensional (3D) structure of proteins, from experimental determination until prediction methods. Protein engineering will also be discussed, with examples from the scientific literature and invited speakers.
Experimental and theoretical approaches linked to the modeling of proteins 3D structures. Limits and possibilities offered by protein engineering.
- Basic introduction about proteins 3D structures
- 3D structure determination of proteins (XRD, NMR) : concepts, applications and examples
- 3D structure prediction
- Conformational changes
- Function prediction for proteins
- 3D structure of membrane proteins
- Protein engineering
A practical course with the cristallisation of a protein and initial analysis by X ray diffraction is proposed
The assesment method will be presented at the first course. It usually consists in two parts :
- a summary of each invited conference, possibly with the analysis of slected article published by the invited speaker
- the presentation of an article about 3D structure and its functional consequences, and the active participation to the presentations made by the other students
A PDF version of the slides is available on the Webcampus site.